Comparative analysis of mechanism of anti-diabetic action of Nishakathakadi Kashayam and Varanadi Kashayam

Abstract

Data collection of phytochemicals present in both Nishakathakadi (NK) and Varanadi (VA) formulations and analyzed which phytochemicals are common between the two, which are unique to each formulation. Additionally, classified the phytochemicals in both formulations. Previous in silico studies showed that both formulations target diabetic-associated genes linked to complications like retinopathy, neuropathy, nephropathy, myopathy, wound healing, and vascular issues. It was found out that NK and VA target 13genes which are similar. Using this information, identified and organized the common phytochemicals in both formulations. To understand the different biological effects due to the overlapped and unique genes targeted by NK and VA, gene ontology and enrichment analysis is performed. These analyses highlighted the biological significance of the targeted genes. The information generated from the enrichment analysis will guide future studies. As part of the comparative analysis, lipase inhibition and cell viability (in intestinal cell lines) were also studied.