Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system

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DC Field	Value	Language
dc.contributor.author	Farooq, Umer	-
dc.contributor.author	Notani, Dimple	-
dc.date.accessioned	2025-03-26T07:08:38Z	-
dc.date.available	2025-03-26T07:08:38Z	-
dc.date.issued	2021-12	-
dc.identifier.uri	http://tdudspace.texicon.in:8080/jspui/handle/123456789/628	-
dc.description.abstract	The CRISPR/Cas9 system is a powerful tool for genome editing and is adaptable for a wide range of applications. Here, we have put together a step-by-step protocol for generating knockout cell lines (coding or non-coding region) using CRISPR/Cas9 tool. The protocol below has been tested on adherent cell lines such as HeLa and MCF7. However, it may easily be adapted to other adherent cell lines with minor variations.	en_US
dc.language.iso	en	en_US
dc.publisher	Cell Press	en_US
dc.subject	CRISPR-Cas9	en_US
dc.subject	Genome	en_US
dc.subject	HeLa	en_US
dc.subject	MCF7	en_US
dc.subject	cell lines	en_US
dc.subject	Plasmids	en_US
dc.title	Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system	en_US
dc.type	Article	en_US
Appears in Collections:	Researcher/Student Publications

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