Please use this identifier to cite or link to this item:
http://tdudspace.texicon.in:8080/jspui/handle/123456789/668| Title: | Optimized protocol for assay for transposaseaccessible chromatin by sequencing (ATACseq) from Drosophila melanogaster brain tissue |
| Authors: | Dhall, Jasmine Kaur Kasturacharya, Nandashree Pandit, Awadhesh C P, Lakshminarayanan |
| Keywords: | transposase-accessible chromatin ATAC-seq Drosophila melanogaster brain tissue |
| Issue Date: | Jun-2023 |
| Publisher: | Cell Press |
| Abstract: | Transposase-accessible chromatin by sequencing (ATAC-seq) has emerged as an advantageous technique to assess chromatin accessibility owing to the robustness of "tagmentation" process and a relatively faster library preparation. A comprehensive ATAC-seq protocol from Drosophilabrain tissue is currently unavailable. Here, we have provided a detailed protocol of ATAC-seq assay from Drosophila brain tissue. Starting from dissection and transposition to amplification of libraries has been elaborated. Furthermore, a robust ATAC-seq analysis pipeline has been presented. The protocol can be easily adapted for other soft tissues. |
| URI: | http://tdudspace.texicon.in:8080/jspui/handle/123456789/668 |
| Appears in Collections: | Researcher/Student Publications |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Optimized protocol for assay for transposase- accessible chromatin by sequencing (ATAC- seq) from Drosophila melanogaster brain tissue.pdf | 1.79 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.